Identification of Novel Chloroplastic Proteins in Arabidopsis
Author Information
Author(s): Sung Tzu-Ying, Chung Tsui-Yun, Hsu Chih-Ping, Hsieh Ming-Hsiun
Primary Institution: Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan
Hypothesis
The ACR11 and ACR12 proteins may function as regulatory proteins related to glutamine metabolism in chloroplasts.
Conclusion
The study identifies four novel ACT domain repeat proteins in Arabidopsis, with ACR11 and ACR12 localized to chloroplasts and co-regulated with GLN2.
Supporting Evidence
- ACR11 and ACR12 proteins are predicted to target to chloroplasts.
- The expression of ACR11 is co-regulated with GLN2, which encodes a chloroplastic glutamine synthetase.
- ACR11 promoter-GUS activity was detected mainly in mature leaves and sepals.
Takeaway
Scientists found new proteins in plants that help with how plants use nitrogen, and these proteins work together with another important protein.
Methodology
The study used protoplast transient expression assays, RNA gel blot analysis, and GUS activity assays in transgenic Arabidopsis.
Digital Object Identifier (DOI)
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