Eradication of Salmonella Typhimurium infection in a murine model of typhoid fever with the combination of probiotic Lactobacillus fermentum ME-3 and ofloxacin
2008

Combining Probiotic and Antibiotic to Fight Salmonella

Sample size: 61 publication Evidence: high

Author Information

Author(s): Truusalu Kai, Mikelsaar Raik-Hiio, Naaber Paul, Karki Tõnis, Kullisaar Tiiu, Zilmer Mihkel, Mikelsaar Marika

Primary Institution: University of Tartu, Estonia

Hypothesis

Can the probiotic Lactobacillus fermentum ME-3 combined with fluoroquinolone therapy eradicate Salmonella Typhimurium infection and improve oxidative stress indices?

Conclusion

The combination of the probiotic L. fermentum ME-3 and ofloxacin effectively eradicated Salmonella Typhimurium and reduced liver and spleen granulomas in a mouse model.

Supporting Evidence

  • The combination treatment eradicated Salmonella Typhimurium from blood, ileum, and liver.
  • Higher counts of intestinal lactobacilli were linked to fewer liver granulomas.
  • The probiotic reduced oxidative stress indices in the ileum mucosa.

Takeaway

This study shows that a special probiotic can help antibiotics work better against a type of bacteria that causes typhoid fever.

Methodology

Mice were infected with Salmonella Typhimurium and treated with either ofloxacin, Lactobacillus fermentum ME-3, or their combination, followed by analysis of bacterial presence and oxidative stress indices.

Limitations

The study did not differentiate between the L. fermentum ME-3 strain and indigenous Lactobacillus strains present in the mice gut.

Participant Demographics

NIH line mice, aged 4 to 6 weeks.

Statistical Information

P-Value

p = 0.0032

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2180-8-132

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