The Chromosomal Passenger Complex and a Mitotic Kinesin Interact with the Tousled-Like Kinase in Trypanosomes to Regulate Mitosis and Cytokinesis
2008

How Trypanosomes Divide: The Role of the Chromosomal Passenger Complex

publication Evidence: moderate

Author Information

Author(s): Li Ziyin, Umeyama Takashi, Wang Ching C.

Primary Institution: Department of Pharmaceutical Chemistry, University of California San Francisco

Hypothesis

The study investigates the interactions of the Chromosomal Passenger Complex and its role in regulating mitosis and cytokinesis in Trypanosomes.

Conclusion

The study concludes that the five-protein complex plays key roles in regulating chromosome segregation and cytokinesis in both forms of Trypanosomes.

Supporting Evidence

  • The study identifies a novel chromosomal passenger complex in Trypanosomes.
  • Time-lapse microscopy confirmed the unique pattern of cytokinesis in both procyclic and bloodstream forms.
  • Knockdown of key proteins disrupted normal cell division processes.

Takeaway

This study shows how a group of proteins helps Trypanosomes divide, which is different from how other cells do it.

Methodology

Time-lapse video microscopy was used to observe the trans-localization of proteins during mitosis and cytokinesis.

Limitations

The experimental conditions may not have been optimal for cell division, potentially affecting the observed time course.

Digital Object Identifier (DOI)

10.1371/journal.pone.0003814

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication