Hyperthermia enhances the reactivation of irradiated adenovirus in HeLa cells
1984
Hyperthermia and Virus Reactivation
publication
Evidence: moderate
Author Information
Author(s): S.M. Piperakis, A.G. McLennan
Primary Institution: University of Liverpool
Hypothesis
Does a brief hyperthermic shock enhance the reactivation of irradiated adenovirus in HeLa cells?
Conclusion
A brief hyperthermic shock significantly enhances the reactivation of irradiated adenovirus in HeLa cells.
Supporting Evidence
- Heating HeLa cells for 10 minutes at 45.5°C enhances virus reactivation by 8-9 fold.
- Maximum reactivation occurs with a 36-hour delay between heating and infection.
- Protein synthesis is required during the first 3 hours after heating for enhanced reactivation.
Takeaway
Heating HeLa cells makes them better at fixing damaged viruses. If you heat them for 10 minutes before giving them the virus, they can fix it much better.
Methodology
HeLa cells were heated at 45.5°C for 10 minutes before being infected with UV-irradiated adenovirus, and their ability to reactivate the virus was measured.
Limitations
The study does not identify the critical target for heat sensitization to irradiation.
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