Deoxycholic Acid and MUC2 Overexpression in Esophageal Cancer Cells
Author Information
Author(s): Wu JianTao, Gong Jun, Geng Juan, Song YinXue
Primary Institution: Department of Gastroenterology, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi, PR China
Hypothesis
Bile acids induce MUC2 expression in human esophageal adenocarcinoma cells through the NF-kB signaling pathway.
Conclusion
Deoxycholic acid induces MUC2 overexpression in human esophageal adenocarcinoma cells by activating the NF-kB transcription pathway.
Supporting Evidence
- Bile acids increased MUC2 protein expression in a dose-dependent manner.
- Nuclear factor-κB activity was also increased with bile acid treatment.
- Aspirin, which inhibits NF-κB, decreased MUC2 transcription.
- PKC was confirmed to be involved in the induction of MUC2 by bile acids.
Takeaway
When certain bile acids are added to esophageal cancer cells, they make the cells produce more of a protein called MUC2, which is important for the cells' function.
Methodology
Human esophageal adenocarcinoma cells were treated with bile acids, and MUC2 expression was measured using luciferase assays and Western blot analysis.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website