Safe Genome Editing in Edible Mushrooms Using CRISPR/Cas9
Author Information
Author(s): Koshi Daishiro, Sugano Junko, Yamasaki Fuga, Kawauchi Moriyuki, Nakazawa Takehito, Oh Minji, Honda Yoichi
Primary Institution: Graduate School of Agriculture, Kyoto University
Hypothesis
Can we develop a genome editing method for Pleurotus ostreatus that avoids the integration of foreign DNA?
Conclusion
The study successfully developed a foreign-DNA-free genome editing method in Pleurotus ostreatus using a trans-nuclei CRISPR/Cas9 approach.
Supporting Evidence
- The method ensures no integration of exogenous DNA in genome-edited strains.
- Successful genome editing was achieved in dikaryotic strains.
- All mutations consisted of small indels at the target site.
Takeaway
Researchers found a way to change the genes in mushrooms without adding any foreign DNA, making it safer for the environment.
Methodology
The study used a trans-nuclei CRISPR/Cas9 system to edit the genome of Pleurotus ostreatus without integrating foreign DNA.
Limitations
The efficiency of the genome editing varied depending on the gRNA used.
Digital Object Identifier (DOI)
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