Mapping Citrullinated Fibrinogen Epitopes in Rheumatoid Arthritis
Author Information
Author(s): van Beers Joyce JBC, Raijmakers Reinout, Alexander Lou-Ella, Stammen-Vogelzangs Judith, Lokate Angelique MC, Heck Albert JR, Schasfoort Richard BM, Pruijn Ger JM
Primary Institution: Radboud University and Utrecht University
Hypothesis
The study aims to systematically map the B-cell response to citrullinated fibrinogen in rheumatoid arthritis.
Conclusion
The study identified three major citrullinated epitope regions in human fibrinogen that are recognized by autoantibodies in rheumatoid arthritis patients.
Supporting Evidence
- The study identified 54 citrullination sites in human fibrinogen.
- Citrullinated fibrinogen is a frequent target of autoantibodies in rheumatoid arthritis patients.
- The method used allows for the simultaneous mapping of modified residues and epitopes.
Takeaway
Researchers found important parts of a protein called fibrinogen that are changed in people with rheumatoid arthritis, which can help in diagnosing the disease.
Methodology
The study used in vitro citrullination of fibrinogen, followed by proteolysis, mass spectrometry, and imaging surface plasmon resonance to analyze patient sera.
Participant Demographics
Sera from rheumatoid arthritis patients were used, with institutional review board approval and informed consent.
Digital Object Identifier (DOI)
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