Clodronate Liposomes Improve Metabolic Profile in Obese Mice
Author Information
Author(s): Feng Bin, Jiao Ping, Nie Yaohui, Kim Thomas, Jun Dale, van Rooijen Nico, Yang Zaiqing, Xu Haiyan
Primary Institution: Huazhong Agricultural University, Wuhan, China
Hypothesis
Does peritoneal injection of clodronate liposomes improve insulin sensitivity and reduce macrophage content in diet-induced obese mice?
Conclusion
Clodronate liposomes reduce visceral adipose tissue macrophages and improve insulin sensitivity in diet-induced obese mice.
Supporting Evidence
- Clodronate liposomes significantly reduced blood glucose and insulin levels in DIO mice.
- Systemic glucose tolerance and insulin sensitivity were mildly improved in both lean and DIO mice treated with clodronate liposomes.
- Macrophage content in visceral adipose tissue of DIO mice was effectively decreased without affecting subcutaneous adipose tissue.
- Levels of insulin sensitizing hormone adiponectin were significantly elevated in circulation.
Takeaway
Injecting special liposomes can help fat mice get better at using sugar and reduce bad cells in their fat.
Methodology
Mice were injected with clodronate liposomes to deplete macrophages, and various metabolic parameters were assessed.
Potential Biases
Potential bias due to the specific targeting of macrophages in visceral fat without affecting subcutaneous fat.
Limitations
The beneficial effects were mild and may be influenced by increased lipolysis.
Participant Demographics
Male C57BL/6J mice, aged 3 weeks, used for the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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