Kbus/Idr Mouse Strain: A New Model for Studying Bone and Phosphate Disorders
Author Information
Author(s): Moriyama Kenji, Hanai Atsuko, Mekada Kazuyuki, Yoshiki Atsushi, Ogiwara Katsueki, Kimura Atsushi, Takahashi Takayuki
Primary Institution: Mukogawa Women's University
Hypothesis
The Kbus/Idr mouse strain carries a novel mutation in the Phex gene that leads to skeletal abnormalities and hypophosphatemia.
Conclusion
The Kbus mouse model is a valuable tool for understanding the mechanisms of bone mineralization and phosphate homeostasis.
Supporting Evidence
- Kbus/Idr mice exhibited bone mineralization defects inherited in an X chromosome-linked dominant manner.
- RT-PCR experiments identified a novel mutation in the Phex gene associated with hypophosphatemic rickets.
- Alkaline phosphatase activity was significantly higher in Kbus/Idr mice compared to control mice.
Takeaway
Scientists created a special mouse that has bone problems and low phosphate levels, which helps them learn more about similar issues in humans.
Methodology
Histopathological and X-ray examinations, RT-PCR, and biochemical assays were performed to characterize the Kbus/Idr mouse strain.
Limitations
The study primarily focused on skeletal abnormalities without exploring other potential health impacts of the mutation.
Participant Demographics
Mice were highly inbred Kbus/Idr strains maintained for over 20 generations.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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