Metastatic susceptibility locus, an 8p hot-spot for tumour progression disrupted in colorectal liver metastases: 13 candidate genes examined at the DNA, mRNA and protein level
2008

Genes on Chromosome 8p and Colorectal Cancer Progression

Sample size: 44 publication Evidence: moderate

Author Information

Author(s): Donia P. Macartney-Coxson, Kylie A. Hood, Hong-jun Shi, Teresa Ward, Anna Wiles, Rosemary O'Connor, David A. Hall, Rod A. Lea, Janice A. Royds, Richard S. Stubbs, Serena Rooker

Primary Institution: Wakefield Gastroenterology Research Institute, Wellington, New Zealand

Hypothesis

The study investigates the role of genes on chromosome 8p in colorectal cancer progression and metastasis.

Conclusion

The study identifies a region on chromosome 8p that is disrupted in colorectal cancer, suggesting it plays a significant role in tumor progression.

Supporting Evidence

  • 73% of samples showed loss of heterozygosity in the 8p region.
  • 8 out of 11 liver metastasis samples had loss not present in matched primary colon tumors.
  • ADAMDEC1 expression decreased during tumor progression.
  • Increased STC1 and LOXL2 expression occurred during tumorigenesis.
  • Low DcR1/TNFRSF10C expression was linked to extrahepatic metastases.

Takeaway

Researchers looked at genes on a specific part of chromosome 8 to see how they affect colorectal cancer, finding that many of these genes are important for cancer growth.

Methodology

The study used loss of heterozygosity analyses, mutation screening, and gene expression analysis on clinical samples.

Potential Biases

Potential biases in sample selection and analysis methods could affect results.

Limitations

The study may not account for all genetic variations affecting colorectal cancer progression.

Participant Demographics

Patients included 44 sporadic colorectal cancer patients, with some having matched normal and tumor samples.

Statistical Information

P-Value

p = 0.005 for low DcR1/TNFRSF10C mRNA expression and extrahepatic metastases; p = 0.02 for SNP rs4872077 association.

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2407-8-187

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