How CRABP2 Affects Gene Regulation in Cancer
Author Information
Author(s): Francesca Corlazzoli, Stefano Rossetti, Gaia Bistulfi, Ren Mingqiang, Nicoletta Sacchi
Primary Institution: Cancer Genetics Program, Roswell Park Cancer Institute, Buffalo, New York, United States of America
Hypothesis
Inheritable aberrant chromatin states in cancer and aging are caused by genetic/environmental factors.
Conclusion
Interference with CRABP2 function induces epigenetic repression of a RA-regulated gene network downstream of RARα, leading to significant biological outcomes.
Supporting Evidence
- CRABP2 is critical for transporting retinoic acid to RARα.
- Knockdown of CRABP2 leads to transcriptional repression of RA-responsive genes.
- The chromatin state becomes unresponsive to retinoic acid after CRABP2 interference.
- Hypermethylation of RARβ2 and CRBP1 correlates with their silencing.
- Impaired CRABP2 function results in loss of acinar morphogenesis in breast epithelial cells.
Takeaway
When a protein called CRABP2 doesn't work right, it can cause important genes to be turned off in a way that can be passed down to future cells.
Methodology
The study involved targeting CRABP2 in human mammary epithelial cells to observe effects on gene expression and chromatin state.
Participant Demographics
Human mammary epithelial cells were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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