Crystal Structure of an Active Form of Human MMP-1
2006

Structure of Active Human MMP-1

publication Evidence: high

Author Information

Author(s): Shalini Iyer, Robert Visse, Hideaki Nagase, K. Ravi Acharya

Primary Institution: University of Bath and Imperial College London

Hypothesis

The study aims to elucidate the crystal structure of the active form of human MMP-1 to understand its role in collagen degradation.

Conclusion

The crystal structure reveals significant differences between the pro and active forms of MMP-1, providing insights into its catalytic mechanism.

Supporting Evidence

  • The structure was determined at a resolution of 2.67 Å.
  • This is the first MMP-1 structure that is free of inhibitor.
  • A water molecule essential for peptide hydrolysis was observed at the active site.

Takeaway

Scientists looked at the shape of a protein called MMP-1, which helps break down collagen in our bodies, to understand how it works better.

Methodology

The study used X-ray crystallography to determine the structure of the active form of MMP-1.

Digital Object Identifier (DOI)

10.1016/j.jmb.2006.06.079

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