Genome-wide SNP panel for rat studies
Author Information
Author(s): Nijman Isaäc J, Kuipers Sylvia, Verheul Mark, Guryev Victor, Cuppen Edwin
Primary Institution: Hubrecht Institute
Hypothesis
Can a genome-wide SNP panel be effectively used for mapping and association studies in rats?
Conclusion
The study provides a fast and cost-effective platform for genome-wide SNP typing that can be used for genetic mapping and association studies in various rat strains.
Supporting Evidence
- The SNP panel was tested on 38 rats of 34 different strains.
- The panel was used to map an obesity trait in Zucker rats.
- A significant linkage was confirmed to chromosome 5 where the leptin receptor gene is located.
Takeaway
Researchers created a tool to help study genes in rats, which can help us understand diseases better.
Methodology
A genome-wide set of 820 SNP assays was designed and tested on 38 rats from 34 different strains.
Potential Biases
The SNP selection may have been biased towards polymorphisms between specific rat strains.
Limitations
The study did not perform any assay optimization in terms of oligo design or experimental conditions.
Participant Demographics
The study involved 38 rats from 34 different strains, including wild rats.
Digital Object Identifier (DOI)
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