How Partner of Numb Affects Protein Distribution in Drosophila Cells
Author Information
Author(s): Carolina N. L. R. Perdigoto, Louis Gervais, Erin Overstreet, Janice Fischer, Antoine Guichet, François Schweisguth
Primary Institution: Ecole Normale Supérieure, CNRS UMR8542, Paris, France
Hypothesis
Does overexpression of Partner of Numb alter the distribution of the PI4P 5-Kinase Skittles in Drosophila sensory organ precursor cells?
Conclusion
Overexpression of Partner of Numb leads to an asymmetric distribution of Skittles and PIP2 in dividing sensory organ precursor cells.
Supporting Evidence
- Overexpression of Pon alters the distribution of Skittles in dividing SOPs.
- PIP2 and Skittles are uniformly distributed in wild-type SOPs at mitosis.
- RFP::PonLD expression leads to posterior localization of Skittles.
Takeaway
When scientists added too much of a protein called Partner of Numb to certain cells, it changed where another important protein, Skittles, ended up in those cells.
Methodology
The study used live imaging and fluorescent protein reporters to analyze the distribution of PIP2 and Skittles in dividing sensory organ precursor cells.
Potential Biases
Potential bias in interpreting results due to the use of overexpressed proteins as markers.
Limitations
The study could not analyze the phenotypes associated with a loss of sktl activity in the notum due to growth issues in wing imaginal discs.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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