Improving Influenza Vaccine Production Using HEK293 Cells
Author Information
Author(s): Petiot Emma, Jacob Danielle, Lanthier Stephane, Lohr Verena, Ansorge Sven, Kamen Amine A
Primary Institution: Biotechnology Research Institute
Hypothesis
Can perfusion culture improve the production yield of influenza virus in HEK293SF cells?
Conclusion
The study confirms that HEK293SF cells are effective for high yield production of influenza virus, with potential for further improvements.
Supporting Evidence
- HEK293SF cells produced the highest reported titers of infectious viral particles.
- Perfusion culture maintained higher cell viability compared to batch culture.
- Viral production was significantly increased in perfusion mode.
- Cell metabolism was more active in perfusion culture, leading to better yields.
Takeaway
Researchers found that using a special method called perfusion helps make more flu vaccine by keeping the cells healthy and fed.
Methodology
The study compared perfusion and batch culture modes for influenza virus production in HEK293SF cells, analyzing cell metabolism and viral production.
Limitations
The study did not explore all potential metabolic inhibitors that could affect viral production.
Digital Object Identifier (DOI)
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