Vitamin Production and NAD+ Homeostasis in Yeast
Author Information
Author(s): Belenky Peter, Stebbins Rebecca, Bogan Katrina L., Evans Charles R., Brenner Charles
Primary Institution: Dartmouth Medical School
Hypothesis
Is the export of nicotinamide riboside independent of the Nrt1 transporter in yeast?
Conclusion
The study found that both nicotinamide riboside and nicotinic acid are exported metabolites, and their export is independent of their respective transporters.
Supporting Evidence
- Nicotinamide riboside and nicotinic acid are both exported from yeast cells.
- Deletion of the Nrt1 transporter leads to increased export of nicotinamide riboside.
- Extracellular nicotinamide is converted to nicotinic acid in a manner dependent on intracellular nicotinamidase activity.
Takeaway
Yeast can send out certain vitamins into their surroundings, which helps them manage their internal vitamin levels and could be useful for making vitamin supplements.
Methodology
The study involved creating yeast strains with specific gene deletions and measuring the export of NAD+ precursors using bioassays and chromatography.
Limitations
The study primarily focuses on yeast and may not directly translate to other organisms.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website