Human MLPA Probe Design (H-MAPD): a probe design tool for both electrophoresis-based and bead-coupled human multiplex ligation-dependent probe amplification assays
2008

H-MAPD: A Tool for Designing MLPA Probes

publication Evidence: moderate

Author Information

Author(s): Zhi Jizu, Eli Hatchwell

Primary Institution: Genomics Core Facility, School of Medicine, Stony Brook University, Stony Brook, NY, USA

Hypothesis

Can we automate the design of MLPA probes to improve efficiency and reliability?

Conclusion

H-MAPD greatly reduces the efforts for human genomic MLPA probe design.

Supporting Evidence

  • H-MAPD automates the tedious process of MLPA probe design.
  • The software supports both traditional electrophoresis-based and bead-coupled MLPA.
  • H-MAPD is freely available to non-commercial users.

Takeaway

H-MAPD is a computer program that helps scientists quickly create tools to study genes by making it easier to design special probes.

Methodology

The software automates the generation and selection of probes for human genomic MLPA using physical-chemical property tests and uniqueness tests.

Limitations

The software currently only applies to human genomic MLPA probe design and may not account for unexpected factors affecting probe performance.

Digital Object Identifier (DOI)

10.1186/1471-2164-9-407

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