Testing Cefotetan Against Anaerobic Bacteria in Gynecology
Author Information
Author(s): Maurizio Maccato, Gerald Riddle, Sebastian Faro
Primary Institution: Baylor College of Medicine
Hypothesis
Can a more economical and rapid method for anaerobic susceptibility testing be as acceptable as the reference agar dilution method?
Conclusion
The spiral gradient method for testing cefotetan against anaerobic bacteria is as reliable as the reference method and less labor-intensive.
Supporting Evidence
- The spiral gradient technique identified MICs equal to or within one doubling dilution of the reference system in 99.5% of tested organisms.
- The commercial microbroth system identified 96.8% of MICs as equal to or within one doubling dilution of the reference system.
- The disk elution method had only 47% agreement with the agar dilution method for resistant isolates.
Takeaway
This study tested different ways to see how well a medicine called cefotetan works against certain germs. They found that one of the new methods works just as well as the old, complicated way.
Methodology
Five different antibiotic susceptibility methods were used to test cefotetan against anaerobic bacteria isolated from patients with obstetrical or gynecological infections.
Limitations
The disk elution method showed significant variability in labeling organisms as susceptible or resistant, particularly near the breakpoint.
Participant Demographics
Patients with obstetrical or gynecological infections.
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