Construction of an almond linkage map in an Australian population
Author Information
Author(s): Tavassolian Iraj, Rabiei Gholmereza, Gregory Davina, Mnejja Mourad, Wirthensohn Michelle G, Hunt Peter W, Gibson John P, Ford Christopher M, Sedgley Margaret, Wu Shu-Biao
Primary Institution: School of Agriculture, Food and Wine, The University of Adelaide
Hypothesis
Can a moderately saturated linkage map be constructed for almonds using SSRs, SNPs, ISSRs, and RAPDs?
Conclusion
The study successfully constructed a moderately saturated almond linkage map that is highly syntenic and collinear with the Prunus reference map and peach genome.
Supporting Evidence
- The linkage map covered 591.4 cM of the genome with 157 loci.
- The average marker distance of the map was 4.0 cM.
- 17.9% of markers had significantly skewed genotype ratios.
- The map displayed high synteny and colinearity with the Prunus T × E reference map.
Takeaway
Researchers made a map of almond genes to help understand how to grow better almonds in Australia.
Methodology
The study used SSRs, SNPs, ISSRs, and RAPDs to construct linkage maps using One-step and Two-step methods.
Potential Biases
Potential bias due to the skewed segregation ratios observed in some markers.
Limitations
The map coverage was not complete, particularly in linkage group G8.
Participant Demographics
The mapping population consisted of 93 progeny from a cross between the American cultivar 'Nonpareil' and the French cultivar 'Lauranne'.
Statistical Information
P-Value
P < 0.05 for skewed markers
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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