Fluoroquinolone Resistance in Enterobacteriaceae in Italy
Author Information
Author(s): Ilaria Frasson, Antonietta Cavallaro, Cristina Bergo, Sara N Richter, Giorgio Palù
Primary Institution: Department of Histology, Microbiology and Medical Biotechnologies, University of Padua
Hypothesis
The prevalence of aac(6')-Ib-cr plasmid-mediated quinolone resistance determinants was evaluated in enterobacterial isolates.
Conclusion
The study found that the aac(6')-Ib-cr gene was present exclusively in E. coli strains and was associated with high resistance to fluoroquinolones.
Supporting Evidence
- The aac(6')-Ib-cr gene was found in 16 out of 197 samples.
- Most of the clinical strains were clonally related.
- The presence of the aac(6')-Ib-cr gene was confirmed by PCR in both transformants and transconjugants.
Takeaway
This study looked at bacteria from patients in Italy and found a gene that helps some bacteria resist certain antibiotics, especially in E. coli.
Methodology
The presence of the aac(6')-Ib-cr gene was assessed by PCR amplification and sequencing.
Limitations
The study was limited to isolates from a single teaching hospital in Italy.
Participant Demographics
The samples were collected from both inpatients (81%) and outpatients.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website