Comparing Two Mass Spectrometry Techniques for Protein Identification
Author Information
Author(s): Creese Andrew J., Cooper Helen J.
Primary Institution: School of Biosciences, University of Birmingham
Hypothesis
How suitable is electron capture dissociation (ECD) for incorporation within proteomic strategies for the identification of proteins?
Conclusion
LC-CID-MS/MS results in greater overall protein coverage than LC-ECD-MS/MS, but LC-ECD-MS/MS provides longer and more reliable peptide sequence tags.
Supporting Evidence
- LC-CID-MS/MS resulted in greater sequence coverage than LC-ECD-MS/MS: 24% versus 20%.
- ECD produced longer sequence tags than CID for eight out of twelve peptides.
- 62% of lysozyme peptides detected in LC-ECD-MS/MS analyses had sequence tags of ≥6 amino acids.
Takeaway
This study looked at two ways to identify proteins using mass spectrometry. One method was faster and found more proteins, but the other method gave better details about the proteins it did find.
Methodology
The study compared LC-ECD-MS/MS and LC-CID-MS/MS techniques using a six-protein tryptic digest analyzed on a hybrid mass spectrometer.
Limitations
The findings cannot be extrapolated to analyses where CID is performed in the ion trap but detected in the ICR cell.
Digital Object Identifier (DOI)
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