Identifying Tumor Cell Growth Inhibitors Using Zebrafish
Author Information
Author(s): Xiang Jing, Yang Hongbo, Che Chao, Zou Haixia, Yang Hanshuo, Wei Yuquan, Quan Junmin, Zhang Hui, Yang Zhen, Lin Shuo
Primary Institution: Peking University, Beijing, China
Hypothesis
Can a combinatorial chemistry approach identify selective inhibitors of tumor cell growth?
Conclusion
The study successfully identified a novel compound that selectively inhibits CDK2 activity and reduces tumor cell proliferation.
Supporting Evidence
- The identified compound 13-1-e showed the highest potency in inhibiting CDK2 activity.
- Zebrafish embryos were used to rapidly screen for cell cycle inhibitors.
- Compound 13-1-e reduced tumor size in a xenograft model without significant toxicity.
Takeaway
Researchers found a new chemical that can stop cancer cells from growing by testing it on zebrafish embryos.
Methodology
The study used a combinatorial chemistry approach to synthesize small molecules and tested their effects on zebrafish embryos.
Limitations
The lead compound is less potent and selective compared to some previously reported CDK2 inhibitors.
Participant Demographics
Zebrafish embryos were used for testing the compounds.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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