Proteolysis in Breast and Colorectal Cancer
Author Information
Author(s): Garbett E A, Reed M W R, Brown N J
Primary Institution: Professional Unit of Surgery, North Tees General Hospital, Stockton-on-Tees, UK
Hypothesis
The study aims to compare proteolysis in breast and colorectal cancer.
Conclusion
Proteolysis is greater in tumor tissue than in normal tissue for both breast and colorectal cancers.
Supporting Evidence
- Proteolysis is normally tightly regulated and is involved in cancer invasion and metastasis.
- Expression of active MMP-2 and MMP-9 was greater in tumor compared to normal samples.
- Total MMP activity was greater in colorectal tissue than breast tissue samples.
Takeaway
This study found that cancer tissues have more active proteins that help in cancer spread compared to normal tissues.
Methodology
Proteinase and inhibitor expression were analyzed in paired tumor and normal tissue samples from breast and colorectal cancer patients using substrate zymography, Western blotting, and quenched fluorescence substrate hydrolysis.
Participant Demographics
43 breast cancer patients and 24 colorectal cancer patients.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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