Novel RNA-binding properties of the MTG chromatin regulatory proteins
2008
RNA-binding properties of MTG proteins
publication
Evidence: moderate
Author Information
Author(s): Stefano Rossetti, Leontine van Unen, Nicoletta Sacchi, André T. Hoogeveen
Primary Institution: Roswell Park Cancer Institute
Hypothesis
Specific MTG domains may mediate RNA binding.
Conclusion
Both wild type MTGs and chimeric AML1-MTG proteins display in vitro RNA-binding properties, suggesting an RNA component's involvement in MTG-mediated chromatin regulation.
Supporting Evidence
- All MTG proteins can bind RNA.
- The RNA-binding properties can be traced to two regions: the Zinc finger domains in the NHR4 and a novel short basic region upstream of the NHR2.
- Both AML1-MTG fusion proteins display RNA-binding properties.
Takeaway
The MTG proteins can grab onto RNA, which might help them control how genes are turned on or off.
Methodology
RNA-binding assays using synthetic RNA homopolymers and MTG deletion mutants.
Digital Object Identifier (DOI)
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