P130Cas Attenuates Epidermal Growth Factor (EGF) Receptor Internalization by Modulating EGF-Triggered Dynamin Phosphorylation
2011
The Role of p130Cas in EGFR Endocytosis
publication
Evidence: moderate
Author Information
Author(s): Kang Yong Seok, Kim Wook, Huh Yun Hyun, Bae Jeomil, Kim Jin Soo, Song Woo Keun
Primary Institution: Gwangju Institute of Science and Technology
Hypothesis
P130Cas plays a regulatory role in the endocytic regulation of EGFR.
Conclusion
P130Cas inhibits EGFR internalization by interacting with dynamin, thereby stabilizing EGFR at the cell surface.
Supporting Evidence
- P130Cas is required for cell adhesion-induced EGFR phosphorylation.
- Overexpression of p130Cas reduces EGF-induced EGFR internalization.
- Depletion of p130Cas enhances EGFR internalization and degradation.
Takeaway
P130Cas helps keep a protein called EGFR on the cell surface instead of letting it get taken inside the cell, which is important for how cells respond to signals.
Methodology
The study used biochemical quantification, immunoblotting, and coimmunoprecipitation to analyze the effects of p130Cas on EGFR internalization.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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