Unconventional Secretion of Tissue Transglutaminase Involves Recycling Endosomes
Author Information
Author(s): Zemskov Evgeny A., Mikhailenko Irina, Hsia Ru-Ching, Zaritskaya Liubov, Belkin Alexey M.
Primary Institution: University of Maryland School of Medicine
Hypothesis
Recycling endosomes are essential for the externalization of cytoplasmic secretory protein tissue transglutaminase (tTG).
Conclusion
The study reveals that tTG is secreted via a non-classical pathway involving recycling endosomes, rather than the classical ER/Golgi route.
Supporting Evidence
- tTG does not follow the classical ER/Golgi-dependent secretion pathway.
- Blocking recycling endosomes abrogates tTG secretion.
- tTG interacts with internalized β1 integrins inside recycling endosomes.
- Phosphoinositides are required for targeting tTG to endosomal membranes.
Takeaway
This study shows that a protein called tTG leaves cells using a special route that doesn't go through the usual cell parts, like the ER and Golgi. Instead, it uses recycling endosomes to get to the outside of the cell.
Methodology
The researchers used NIH3T3-tTG transfectants to study the kinetics of tTG secretion and employed various pharmacological inhibitors to assess the secretion pathway.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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