Alternative Splicing Dynamics in Myogenesis: A Transcriptomic Atlas for Human Skeletal Muscle Cells
2024
Alternative Splicing Dynamics in Myogenesis: A Transcriptomic Atlas for Human Skeletal Muscle Cells
Sample size: 2
publication
Author Information
Author(s): Donega Stefano, Banskota Nirad, Yang Jen-Hao, Rossi Martina, Tsitsipatis Dimitrios, De Supriyo, Gorospe Myriam, Ferrucci Luigi
Primary Institution: National Institute on Aging
Hypothesis
The study aims to characterize alternative splicing in human myogenesis.
Conclusion
The research identified significant gene expression changes during myogenesis and established a transcriptomic atlas for future studies.
Supporting Evidence
- 3,079 upregulated genes and 2,357 downregulated genes were discovered.
- Gene Set Enrichment Analysis highlighted pathways related to muscle formation and contraction.
- 2,584 myogenesis-dependent isoform-switches were identified.
Takeaway
The study looked at how muscle cells grow and change over time, finding many important genes that help muscles form.
Methodology
The study used two human skeletal muscle cell lines and RNA sequencing over a 5-day differentiation period.
Digital Object Identifier (DOI)
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