Sox9-Haploinsufficiency Causes Glucose Intolerance in Mice
Author Information
Author(s): Claire L. Dubois, Hung Ping Shih, Philip A. Seymour, Nisha A. Patel, James M. Behrmann, Victoria Ngo, Maike Sander
Primary Institution: University of California San Diego
Hypothesis
Does reduced Sox9 gene dosage lead to impaired glucose homeostasis in adult mice?
Conclusion
Sox9-haploinsufficient mice exhibit glucose intolerance due to reduced beta-cell mass.
Supporting Evidence
- Sox9-haploinsufficient mice displayed 50% reduced beta-cell mass at birth.
- Glucose intolerance persisted in Sox9+/Δpan mice at 12 weeks of age.
- Islets from Sox9-haploinsufficient mice exhibited normal insulin secretion when stimulated with glucose.
Takeaway
Mice with less Sox9 gene had trouble keeping their blood sugar normal, especially when they ate a lot of fat.
Methodology
The study used genetic models of Sox9 inactivation in pancreatic progenitor cells and assessed glucose tolerance through intraperitoneal glucose tolerance tests.
Limitations
The study primarily focused on mouse models, which may not fully replicate human conditions.
Participant Demographics
Mice were used in the study, specifically Sox9-haploinsufficient and control littermates.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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