Multiple-locus variable number of tandem repeat analysis (MLVA) of Irish verocytotoxigenic Escherichia coli O157 from feedlot cattle: uncovering strain dissemination routes
2008

Studying E. coli O157 Spread in Cattle

Sample size: 54 publication Evidence: moderate

Author Information

Author(s): Mary Murphy, Donal Minihan, James F Buckley, Micheál O'Mahony, Paul Whyte, Séamus Fanning

Primary Institution: Veterinary Food Safety Laboratory, Cork County Council

Hypothesis

The study aims to identify potential routes of dissemination of E. coli O157 using Multiple-Locus Variable number of tandem repeat Analysis (MLVA).

Conclusion

MLVA is a useful tool for uncovering the dissemination routes of E. coli O157 in the environment and can support improved on-farm management practices.

Supporting Evidence

  • MLVA could separate isolates into clusters, identifying twelve different MLVA types.
  • Strain diversity was observed within pens, individual cattle, and the environment.
  • E. coli O157 was detected in both feed and water troughs, indicating potential transmission routes.

Takeaway

This study looked at how E. coli O157 spreads among cattle and their environment, finding that different strains can be found in various places like water and feed.

Methodology

The study used MLVA to subtype E. coli O157 isolates from environmental and cattle faecal samples collected over four months.

Limitations

The study may not account for all environmental factors influencing E. coli O157 transmission.

Participant Demographics

The study involved cattle from a feedlot in Ireland.

Digital Object Identifier (DOI)

10.1186/1746-6148-4-2

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