High Throughput Interrogation of Somatic Mutations in High Grade Serous Cancer of the Ovary
2011

Study of Mutations in Ovarian Cancer

Sample size: 203 publication Evidence: moderate

Author Information

Author(s): Matulonis Ursula A., Hirsch Michelle, Palescandolo Emanuele, Kim Eejung, Liu Joyce, van Hummelen Paul, MacConaill Laura, Drapkin Ronny, Hahn William C.

Primary Institution: Dana-Farber Cancer Institute

Hypothesis

The study aims to determine the frequency and types of point somatic mutations in high grade serous ovarian cancer (HGSC).

Conclusion

The study found that 56% of tumor samples harbored candidate mutations, indicating that screening for somatic mutations may lead to new therapies for ovarian cancer patients.

Supporting Evidence

  • 56% of tumor samples had candidate mutations.
  • The most common mutations were found in genes like EGFR, KRAS, and TP53.
  • The study suggests that identifying these mutations could lead to new treatment options.

Takeaway

The researchers looked at cancer samples to find changes in genes that could help doctors treat ovarian cancer better.

Methodology

The study used a mutation detection protocol called OncoMap to analyze DNA from formalin-fixed paraffin-embedded tissue samples.

Limitations

Only 'hotspot' mutations were detected, and validation of mutations is necessary; other mutations not included in the OncoMap panel may be missed.

Participant Demographics

Patients with advanced staged HGSC ovarian cancer were selected, excluding those with known BRCA germline mutations.

Digital Object Identifier (DOI)

10.1371/journal.pone.0024433

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