Targeting Aspartate Aminotransferase in Breast Cancer
Author Information
Author(s): Joshua Marshall Thornburg, Kristin K Nelson, Brian F Clem, Andrew N Lane, Arumugam Sengodagounder, Allan Simmons, John W Eaton, Sucheta Telang, Jason Chesney
Primary Institution: University of Louisville
Hypothesis
Oxamate may disrupt the metabolism and growth of breast adenocarcinoma cells.
Conclusion
AAT may be a valid molecular target for the development of anti-neoplastic agents.
Supporting Evidence
- Oxamate and AOA decreased the growth of MDA-MB-231 cells in vitro.
- Oxamate reduced oxygen consumption by 61% in treated cells.
- Ectopic expression of AAT conferred resistance to the anti-proliferative effects of oxamate.
- Selective inhibition of AAT decreased MDA-MB-231 cell proliferation.
Takeaway
Researchers found that a substance called oxamate can slow down the growth of breast cancer cells by affecting their energy production.
Methodology
The study examined the effects of oxamate and AOA on breast cancer cells and normal cells, measuring glucose utilization, oxygen consumption, and cell proliferation.
Participant Demographics
The study involved MDA-MB-231 breast adenocarcinoma cells and normal human mammary epithelial cells.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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