Stability of Reference Genes in Canine Tissues
Author Information
Author(s): Duncan Ayers, Dylan N. Clements, Fiona Salway, Philip J. R. Day
Primary Institution: University of Manchester
Hypothesis
Some of the genes selected would demonstrate stable expression in the different canine connective tissues investigated.
Conclusion
The reference genes demonstrating the most stable expression within each different canine articular tissue were identified, but no single reference gene was identified as having stable expression in all different tissue types.
Supporting Evidence
- The optimal reference genes for comparing gene expression data between normal and diseased infrapatella fat pad were RPL13A and YWHAZ.
- The ideal reference genes for comparing normal and osteoarthritic cartilage were RPL13A and SDHA.
- The best reference genes for comparing normal and ruptured cranial cruciate ligament were B2M and TBP.
Takeaway
This study looked at which genes are best to use as a reference when studying gene expression in dog tissues, finding that different tissues need different reference genes.
Methodology
Real-time reverse transcription PCR and a mathematical algorithm were used to evaluate the stability of 9 reference genes in normal and diseased canine articular tissues.
Limitations
No single reference gene was identified as having stable expression across all tissue types.
Participant Demographics
Canine samples from healthy and diseased dogs.
Digital Object Identifier (DOI)
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