A Novel Role of the NRF2 Transcription Factor in the Regulation of Arsenite-Mediated Keratin 16 Gene Expression in Human Keratinocytes
2008
NRF2's Role in Arsenic-Induced Keratin 16 Expression
publication
Evidence: high
Author Information
Author(s): Hitoshi Sugioka, Yoshihiko Nakagi, Yoshihiko Saijo, Yasuaki Yoshida, Takahiko Endo
Primary Institution: Asahikawa Medical College
Hypothesis
Does NRF2 mediate the transcriptional activation of the K16 gene in human keratinocytes exposed to inorganic sodium arsenite?
Conclusion
The study shows that NRF2 activation is crucial for the induction of the K16 gene in human keratinocytes by arsenic.
Supporting Evidence
- Arsenic exposure increases K16 gene expression in skin cells.
- NRF2 translocates to the nucleus and binds to the K16 gene promoter.
- The study identifies a novel mechanism of NRF2 in skin disorders.
Takeaway
When skin cells are exposed to arsenic, a protein called NRF2 helps turn on a gene that can lead to skin problems.
Methodology
The study used reverse transcriptase polymerase chain reaction, luciferase assays, Western blots, and electrophoretic mobility shift assays to analyze gene expression.
Statistical Information
P-Value
<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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