Activation of the Wnt/β-Catenin Signaling Pathway by Mechanical Ventilation Is Associated with Ventilator-Induced Pulmonary Fibrosis in Healthy Lungs
2011

Mechanical Ventilation and Lung Injury

Sample size: 18 publication Evidence: moderate

Author Information

Author(s): Villar Jesús, Cabrera Nuria E., Valladares Francisco, Casula Milena, Flores Carlos, Blanch Lluís, Quilez María Elisa, Santana-Rodríguez Norberto, Kacmarek Robert M., Slutsky Arthur S.

Primary Institution: CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain

Hypothesis

The Wnt/β-catenin signaling plays a role during ventilator-induced lung injury (VILI).

Conclusion

Mechanical ventilation with high tidal volumes activates the Wnt/β-catenin signaling pathway, which may contribute to lung injury and fibrosis.

Supporting Evidence

  • High tidal volume mechanical ventilation caused significant lung inflammation and fibrosis.
  • WNT5A and non-phosphorylated β-catenin levels were significantly higher in the high tidal volume group.
  • The study demonstrated that even healthy lungs can be damaged by mechanical ventilation.

Takeaway

When doctors use machines to help people breathe, using too much air can hurt the lungs and cause scarring.

Methodology

The study used a randomized controlled animal model with adult male Sprague-Dawley rats, comparing low and high tidal volume mechanical ventilation.

Limitations

The study did not use WNT5A deficient animals to confirm the role of the Wnt/β-catenin pathway in VILI.

Participant Demographics

Adult, healthy, male Sprague-Dawley rats.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0023914

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