Fingerprinting Mycobacterium tuberculosis in Kerala using AFLP and IS6110-RFLP
Author Information
Author(s): Krishnan Manju Y, Radhakrishnan Indulakshmi, Joseph Biljo V, GK Madhavi Latha, Kumar R Ajay, Mundayoor Sathish
Primary Institution: Rajiv Gandhi Centre for Biotechnology
Hypothesis
Can Amplified Fragment Length Polymorphism (AFLP) effectively differentiate clinical isolates of Mycobacterium tuberculosis in Kerala?
Conclusion
AFLP alone has limited use in fingerprinting Mycobacterium tuberculosis isolates in Kerala, but combined with IS6110-RFLP, it shows better differentiation of high copy isolates.
Supporting Evidence
- AFLP showed maximum polymorphism with the EO/MC primer pair among clinical isolates.
- 85% of low copy IS6110 isolates clustered together, indicating limited diversity.
- AFLP profiles for non-tuberculous mycobacteria were highly different from those of M. tuberculosis.
Takeaway
The study looked at how well two methods could tell different types of tuberculosis germs apart. One method worked better for some germs than others.
Methodology
Fifty clinical isolates of M. tuberculosis were analyzed using AFLP and IS6110-RFLP techniques.
Limitations
AFLP alone does not efficiently differentiate low copy isolates of M. tuberculosis.
Participant Demographics
Clinical isolates from tuberculosis patients in Kerala, South India.
Digital Object Identifier (DOI)
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