Diversity of Raft-Like Domains in Late Endosomes
2007

Diversity of Raft-Like Domains in Late Endosomes

publication Evidence: moderate

Author Information

Author(s): Sobo Komla, Chevallier Julien, Parton Robert G., Gruenberg Jean, van der Goot F. Gisou

Primary Institution: University of Geneva

Hypothesis

Are late endosomal membranes sub-compartmentalized into membrane microdomains?

Conclusion

Late endosomes contain cholesterol-rich microdomains that differ in composition and properties.

Supporting Evidence

  • Late endosomes contain raft-type membrane domains.
  • These domains vary in protein composition and properties.
  • Cholesterol-rich microdomains are present on both the limiting membrane and intraluminal vesicles.

Takeaway

Late endosomes, which help transport materials in cells, have special areas rich in cholesterol that help them work better.

Methodology

Sub-organellar fractionation techniques combined with electron microscopy were used to analyze late endosomal membranes.

Digital Object Identifier (DOI)

10.1371/journal.pone.0000391

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