Molecular Typing of Multidrug-Resistant Salmonella Blockley Outbreak Isolates from Greece
Author Information
Author(s): Panayotis T. Tassios, Christos Chadjichristodoulou, Maria Lambiri, Athina Kansouzidou-Kanakoudi, Zannina Sarandopoulou, Jenny Kourea-Kremastinou, Leonidas S. Tzouvelekis, Nicholas J. Legakis
Primary Institution: University of Athens, Athens, Greece
Hypothesis
The study aims to characterize the antibiotic resistance phenotypes and DNA fingerprints of Salmonella Blockley outbreak isolates in Greece.
Conclusion
The study found that two distinct DNA fingerprints of Salmonella Blockley were associated with different antibiotic resistance phenotypes.
Supporting Evidence
- S. Blockley accounted for seven of the 13,199 salmonella isolates identified in Greece from 1976 to 1997.
- High resistance rates were observed to tetracycline (100%), streptomycin and kanamycin (90%), chloramphenicol (83%), and nalidixic acid (52%).
- Two dominant multidrug-resistance phenotypes were associated with two distinct DNA fingerprints.
- Most outbreak isolates were grouped in subtypes A2 and A4, consisting of 11 and 12 isolates, respectively.
Takeaway
Researchers looked at sick people in Greece and found two types of Salmonella Blockley that were resistant to many medicines.
Methodology
The study used pulsed-field gel electrophoresis to analyze DNA fingerprints and tested antibiotic susceptibility using disk diffusion methods.
Limitations
The study did not confirm a common source for the outbreak despite the geographic spread of cases.
Participant Demographics
Isolates were from human cases of gastroenteritis in Greece.
Statistical Information
P-Value
0.005
Statistical Significance
p<0.05
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