Genetic Instability in Prostate Cancer Cells
Author Information
Author(s): Thuret R, Chantrel-Groussard K, Azzouzi A-R, Villette J-M, Guimard S, Teillac P, Berthon P, Houlgatte A, Latil A, Cussenot O
Primary Institution: CeRePP-EA3104, University Paris 7, France
Hypothesis
Can genetic lesions like loss of heterozygosity (LOH) be detected in prostatic cells obtained by prostatic massage during early diagnosis of prostate cancer?
Conclusion
The presence of LOH in urinary prostatic cells obtained after prostatic massage is significantly associated with prostate cancer on biopsy.
Supporting Evidence
- One or more allelic deletions were found in prostatic fluid from 57 patients, of whom 33 (58%) had prostate cancer.
- Sensitivity and specificity of LOH detection were 86.7% and 44%, respectively.
- Analysis of LOH from prostatic tumors revealed similar patterns compared to prostatic fluid cells in 86% of cases.
Takeaway
Doctors can find signs of prostate cancer in urine samples taken after a special massage, which helps catch the disease early.
Methodology
Prostatic cells were collected from 99 patients and analyzed for LOH on six chromosomal regions using quantitative RT-PCR and DNA analysis.
Limitations
The study had a small number of prostate specimens available for analysis, limiting the clinical significance of the findings.
Participant Demographics
Mean age of participants was 65.4 years, with a PSA range of 4 to 10 ng/ml.
Statistical Information
P-Value
0.002
Statistical Significance
p=0.002
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website