Comparative Study of Extraction Methods for Phytochemicals from Rheum tataricum L.
Author Information
Author(s): Amangeldinova Madina, Ersatır Mehmet, Necip Adem, Yilmaz Mustafa Abdullah, Cimentepe Mehmet, Kudrina Nataliya, Terletskaya Nina V., Ozturk Cimentepe Ozge, Yildirim Metin
Primary Institution: Al-Farabi Kazakh National University
Hypothesis
What are the optimal extraction methods for isolating biologically active compounds from Rheum tataricum L. roots?
Conclusion
Ultrasound-assisted extraction proved to be the most effective method for extracting phytochemicals with strong antioxidant and antibacterial properties from Rheum tataricum L. roots.
Supporting Evidence
- The ultrasound-assisted extraction method yielded the highest total phenolic content of 213.44 mg GAE/mL.
- Extracts showed antibacterial activity against both Gram-positive and Gram-negative bacteria with MIC values ranging from 31.25 to 250 μg/mL.
- Ultrasound extraction demonstrated superior antioxidant properties compared to other methods.
Takeaway
This study found that using ultrasound to extract plant compounds is better than other methods, making it easier to get useful ingredients from the rhubarb plant.
Methodology
The study utilized supercritical CO2, subcritical ethanol, and ultrasound-assisted extraction methods to obtain extracts from Rheum tataricum L. roots, followed by LC-MS/MS analysis to quantify phytochemicals and evaluate antioxidant and antibacterial activities.
Limitations
The study did not explore the long-term stability of the extracted compounds or their effects in vivo.
Statistical Information
P-Value
0.0173 to 0.0400 mg/mL
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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