Epigenetic Changes in Response to Severe Burn Injury
Author Information
Author(s): Arias-Pérez Osvaldo, Escobedo-Tapia Thelma, Cintora-Ahumada Cecilia, León-Solís Lizbel, Leyva-García Norberto, Aréchaga-Ocampo Elena, Franco-Cendejas Rafael, Hernández-Hernández Oscar, Suárez-Sánchez Rocío, Kamińska Joanna, Tylicka Marzena, Koper-Lenkiewicz Olga
Primary Institution: Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra Ibarra”, Mexico City, Mexico
Hypothesis
The study aims to unveil the histone H3 posttranslational modifications profile and their enrichment in promoters of inflammatory genes in response to severe burns.
Conclusion
Severe burns trigger epigenetic changes and differential H3 PTM enrichment at inflammation gene promoters.
Supporting Evidence
- Severe burns activate systemic inflammation and increase cytokine levels.
- Eight H3 PTMs decreased at 5 days post-burn.
- Burn patients showed decreased enrichment of H3K9me2 in certain inflammatory gene promoters.
- H3S28p enrichment diminished during the first 10 days post-burn.
- Septic burn patients exhibited increased enrichment of H3K9me2 in inflammatory gene promoters.
Takeaway
When people get severe burns, their body changes how it controls inflammation at the genetic level, which might lead to complications like sepsis.
Methodology
The study analyzed histone H3 posttranslational modifications in blood samples from burn patients using ELISA and ChIP assays.
Potential Biases
Potential bias due to lack of analysis of variables such as gender, medication, and baseline inflammation status.
Limitations
The modest sample size may limit the analysis and prevent the examination of factors like burn etiology and patient age.
Participant Demographics
Twelve male patients over 18 years old with severe burns and six healthy male volunteers.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website