Reovirus Membrane Penetration and Apoptosis Regulation
Author Information
Author(s): Danthi Pranav, Coffey Caroline M., Parker John S. L., Abel Ty W., Dermody Terence S.
Primary Institution: Vanderbilt University School of Medicine
Hypothesis
Membrane penetration by reovirus results in the delivery of the μ1 ϕ domain into the cytosol where it activates a cellular trigger to induce apoptosis.
Conclusion
The μ1 ϕ domain of reovirus plays a critical role in both membrane penetration and the induction of apoptosis following infection.
Supporting Evidence
- The μ1 ϕ domain is sufficient to elicit a cell death response.
- Mutations in the μ1 ϕ domain diminish reovirus membrane penetration efficiency.
- Apoptosis-defective ϕ mutant viruses display diminished neurovirulence following intracranial inoculation of newborn mice.
- The study highlights an important role for the μ1 ϕ domain in both membrane penetration and apoptosis induction.
Takeaway
Reovirus can enter cells and cause them to die, and a specific part of the virus helps it do both things.
Methodology
The study involved generating mutant viruses and analyzing their capacity to penetrate membranes and induce apoptosis in cultured cells and in newborn mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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