Stability of Fluorescent Signals on Antibody Microarrays
Author Information
Author(s): Gu Qiang, Sivanandam Thamil Mani, Kim Caroline Aehyun
Primary Institution: Wake Forest University School of Medicine
Hypothesis
How do fluorescent signals on antibody microarrays change over time when stored at -20°C?
Conclusion
Microarray slides can be preserved and rescanned multiple times using a confocal laser scanner over a period of days or weeks without significant changes in signal intensity.
Supporting Evidence
- Fluorescent intensities did not show significant changes over time.
- Microarray slides can be stored at -20°C without losing signal quality.
- Repeated scans did not lead to saturation of microarray spots.
Takeaway
If you keep special slides in the freezer, you can take pictures of them later without losing the colors.
Methodology
Microarray slides were scanned at day 0, 10, 20, and 30 after being stored at -20°C, and signal intensities were measured using a confocal laser scanner.
Potential Biases
Potential instability of laser power and PMT settings could affect results.
Limitations
The study only tested Cy3 and Cy5 dyes, and results may not apply to other fluorescent dyes.
Participant Demographics
Proteins extracted from young and adult mice.
Statistical Information
P-Value
p > 0.05
Statistical Significance
p > 0.05
Digital Object Identifier (DOI)
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