Identifying KSRP Target Transcripts Affected by PI3K-AKT Signaling
Author Information
Author(s): Ruggiero Tina, Trabucchi Michele, Ponassi Marco, Corte Giorgio, Chen Ching-Yi, al-Haj Latifa, Khabar Khalid SA, Briata Paola, Gherzi Roberto
Primary Institution: Istituto Nazionale per la Ricerca sul Cancro (IST), Genova, Italy
Hypothesis
PI3K-AKT activation could regulate the expression of transcripts additional to β-catenin by targeting KSRP.
Conclusion
The study identified several transcripts that are targets of KSRP and whose expression is increased by PI3K-AKT activation.
Supporting Evidence
- 34 ARE-containing transcripts were identified as being upregulated in cells expressing a constitutively active form of AKT1.
- Seven mRNAs were found to be upregulated in cells expressing activated AKT1.
- KSRP knock-down or PI3K-AKT activation consistently increased the steady-state levels and stability of the identified KSRP targets.
Takeaway
This study found that a protein called KSRP helps control the stability of certain mRNAs, and when a signaling pathway called PI3K-AKT is activated, it makes these mRNAs more stable.
Methodology
Microarray analyses and affinity chromatography were used to identify KSRP target transcripts in pituitary αT3-1 cells.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website