Rasd1 Modulates the Coactivator Function of NonO in the Cyclic AMP Pathway
2011
Rasd1 and NonO Interaction in the cAMP Pathway
publication
10 minutes
Evidence: moderate
Author Information
Author(s): Ong Shufen Angeline, Tan Jen Jen, Tew Wai Loon, Chen Ken-Shiung
Primary Institution: Nanyang Technological University, Singapore
Hypothesis
Rasd1 modulates the coactivator function of NonO in the cyclic AMP pathway.
Conclusion
Rasd1 interacts with NonO to suppress the transcription of specific CREB target genes, NR4A1 and NR4A2.
Supporting Evidence
- NonO was identified as a novel interacting partner of Rasd1 via affinity pull-down assay.
- Rasd1 and NonO co-localize in the nucleus and regulate the cAMP-dependent pathway.
- Rasd1 represses CREB-mediated transcription in a dose-dependent manner.
Takeaway
Rasd1 and NonO work together to help control how certain genes are turned on or off in response to signals in the body.
Methodology
The study used affinity pulldown, co-immunoprecipitation, and reporter gene assays to investigate the interaction between Rasd1 and NonO.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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