Mutation Analysis of the p73 Gene in Nonastrocytic Brain Tumours
Author Information
Author(s): Alonso M E, Bello M J, Gonzalez-Gomez P, Lomas J, Arjona D, Campos J M de, Kusak M E, Sarasa J L, Isla A, Rey J A
Primary Institution: Laboratorio de Oncogenética Molecular, Departamento de Cirugia Experimental, Unidad de Investigación, Hospital Universitario La Paz, Madrid, 28046, Spain
Hypothesis
Is the p73 gene involved in the development of nonastrocytic brain tumours?
Conclusion
The study found that while loss of heterozygosity at 1p36 and p73 sequence changes were observed, establishing a causal role of p73 variations in nonastrocytic brain tumours is challenging.
Supporting Evidence
- Loss of heterozygosity at 1p36 occurs frequently in nonastrocytic brain tumours.
- p73 is a candidate for the tumour suppressor gene targeted by the deletion.
- LOH was found in about 50% of cases, primarily in oligodendroglial tumours and meningiomas.
- A missense mutation in p73 was identified in one primary lymphoma.
Takeaway
Researchers looked at brain tumours to see if a gene called p73 was involved, but they found it hard to prove that changes in this gene caused the tumours.
Methodology
The study analyzed 65 tumour samples for p73 alterations using PCR-SSCP and direct DNA sequencing.
Limitations
It is difficult to establish a causal role of the p73 variations in nonastrocytic brain tumours development.
Digital Object Identifier (DOI)
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