The role of a new CD44st in increasing the invasion capability of the human breast cancer cell line MCF-7
2011
New CD44st Increases Invasion in Breast Cancer Cells
publication
Evidence: moderate
Author Information
Author(s): Fang Xin Jian, Jiang Hua, Zhao Xv Peng, Jiang Wei Mei
Primary Institution: The second People's Hospital of Lianyungang
Hypothesis
Does the new CD44st isoform increase the invasion capability of MCF-7 breast cancer cells?
Conclusion
The new CD44st isoform enhances the invasion capability of MCF-7 cells through the Ras/MAPK signaling pathway.
Supporting Evidence
- CD44st was not expressed in sensitive MCF-7 cells but was present in multidrug resistant MCF-7/Adr cells.
- HA treatment activated MMP-2 and MMP-9 secretion in MCF-7/CD44st cells.
- Blocking CD44 with antibodies reduced the invasion capability of MCF-7/CD44st cells.
Takeaway
A new version of a protein called CD44 helps breast cancer cells move and spread more easily. This happens when it interacts with another substance called hyaluronan.
Methodology
The study used RT-PCR and Western blotting to analyze CD44st expression and its effects on cell invasion through various assays.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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