Identifying Proteins in Biofilms of Haemophilus influenzae
Author Information
Author(s): Timothy K Gallaher, Siva Wu, Paul Webster, Rodrigo Aguilera
Primary Institution: University of Southern California
Hypothesis
What are the protein components of the biofilm formed by non-typeable Haemophilus influenzae?
Conclusion
The study identified 265 unique proteins associated with the biofilm structure of non-typeable Haemophilus influenzae.
Supporting Evidence
- 265 proteins were identified in the extracellular matrix sample.
- The most over-represented COG groups were related to cell motility and secretion.
- A number of hypothetical or uncharacterized proteins were observed.
Takeaway
Researchers found many proteins that help form a slimy layer around bacteria, which makes them hard to kill with antibiotics.
Methodology
Proteomic analysis via LC-MS/MS was used to identify proteins from an ex-vivo biofilm of non-typeable Haemophilus influenzae.
Limitations
The presence of lysed cell components cannot be ruled out, and it is unclear if some identified proteins contribute to biofilm structure.
Digital Object Identifier (DOI)
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