Gene Expression in Leukemia Cells and Normal Leukocytes
Author Information
Author(s): French Deborah, Yang Wenjian, Hamilton Leo H., Neale Geoffrey, Fan Yiping, Downing James R., Cox Nancy J., Pui Ching-Hon, Evans William E., Relling Mary V.
Primary Institution: St. Jude Children's Research Hospital
Hypothesis
Expression that is concordant across tissues is more likely influenced by genetic variability than gene expression which is discordant between tissues.
Conclusion
Genes whose expression is concordant among tissue types are more likely to be associated with germline cis-SNPs than genes with discordant expression.
Supporting Evidence
- 176 genes showed significant positive correlation in expression between leukemia cells and normal leukocytes.
- 20 of these 176 genes were significantly related to cis-SNP genotypes.
- Validation in independent patient sets confirmed the association of gene expression with cis-SNPs.
Takeaway
This study looked at how genes behave in leukemia cells compared to normal blood cells and found that genes that act similarly in both are often influenced by genetic differences.
Methodology
Gene expression was quantified for 11,783 genes in paired samples of leukemia cells and normal leukocytes, and genetic variation was assessed at over 500,000 SNPs.
Potential Biases
Potential biases may arise from the selection of patient samples and the methods used for gene expression analysis.
Limitations
The study is limited to specific patient populations and may not generalize to all individuals with leukemia.
Participant Demographics
Patients with newly diagnosed childhood acute lymphoblastic leukemia.
Statistical Information
P-Value
p<0.008
Statistical Significance
p<0.008
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website