How HIV-1 Co-Receptor Binding Site Antibodies Are Made
Author Information
Author(s): Forsell Mattias N. E., Dey Barna, Mörner Andreas, Svehla Krisha, O'dell Sijy, Högerkorp Carl-Magnus, Voss Gerald, Thorstensson Rigmor, Shaw George M., Mascola John R., Karlsson Hedestam Gunilla B., Wyatt Richard T.
Primary Institution: Vaccine Research Center, National Institutes of Health, Bethesda, Maryland, United States of America
Hypothesis
The presence of primate CD4 is required for the elicitation of co-receptor site antibodies by HIV-1 envelope glycoprotein trimers.
Conclusion
The study shows that the presence of primate CD4 is necessary for the elicitation of antibodies targeting the HIV-1 co-receptor binding site.
Supporting Evidence
- Antibodies against the co-receptor binding site are generated during natural HIV infection.
- Immunization with Env trimers in primates leads to the production of CD4i antibodies.
- CD4i antibodies were detected in sera from monkeys but not in wild-type rabbits.
Takeaway
This study found that to make certain antibodies against HIV, the body needs a special helper called CD4. Without it, the antibodies can't be made.
Methodology
The study involved immunizing rabbits, monkeys, and human-CD4 transgenic rabbits with envelope glycoprotein trimers and analyzing the resulting antibody responses.
Limitations
The study primarily focused on specific animal models and may not fully represent human responses.
Participant Demographics
The study included cynomolgus macaques and rabbits, including transgenic rabbits expressing human CD4.
Digital Object Identifier (DOI)
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