Role of receptor polymorphism and glycosylation in virus infection
Author Information
Author(s): Yan Yuhe, Jung Yong T, Wu Tiyun, Kozak Christine A
Primary Institution: National Institute of Allergy and Infectious Diseases
Hypothesis
The study examines how receptor variants and glycosylation affect the infection and cytopathicity of ecotropic mouse gammaretroviruses.
Conclusion
Virus entry and syncytium formation are influenced by specific amino acid residues in the receptor and virus Env, as well as by glycosylation of cellular proteins.
Supporting Evidence
- The study identified critical amino acid substitutions in the receptor that affect virus binding.
- Glycosylation patterns of the receptor were shown to influence virus entry.
- Different cell lines expressing the same receptor variant exhibited varying susceptibility to virus-induced cytopathicity.
Takeaway
The way viruses enter cells and cause them to fuse together depends on tiny changes in the virus and the cell's receptor, and how the cell's proteins are modified.
Methodology
The study involved expressing different receptor variants in non-susceptible cells and evaluating their susceptibility to virus infection and syncytium formation.
Limitations
The study does not address all potential factors influencing virus entry and cytopathicity beyond receptor and glycosylation variations.
Digital Object Identifier (DOI)
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